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Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks

Received: 19 April 2019     Published: 15 June 2019
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Abstract

In this study, a simple yet effective protein detection method was established by using bimetallic Au@Ag core-shell nanobrick as SERS active building blocks. The Au@Ag core-shell nanobrick is synthesized by well-defined ‘two-step’ growing method, enabling uniform and controllable silver shell growth on the surface of the gold nanorods induced by surfactant replacement. The thickness of the silver shell can be programmably adjusted by controlling the amount of silver nitrate in the system, which in turn realized the controllable adjustment of the optical properties of Au@Ag core-shell nanobrick. We selected bovine serum albumin as a model protein and established a highly-sensitive assay for label-free SERS detection of protein. The detection limit can reach to as low as 0.5 μg/mL, enabling promising avenue for its clinical application in ultrasensitive biomarker detection.

Published in Science Discovery (Volume 7, Issue 3)
DOI 10.11648/j.sd.20190703.13
Page(s) 147-151
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Plasmonic Nanoparticle, Au@Ag Core-shell Nanobrick, SERS, Label-Free Protein Detection

References
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  • APA Style

    Huang Liu, Yi Chen. (2019). Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Science Discovery, 7(3), 147-151. https://doi.org/10.11648/j.sd.20190703.13

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    ACS Style

    Huang Liu; Yi Chen. Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Sci. Discov. 2019, 7(3), 147-151. doi: 10.11648/j.sd.20190703.13

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    AMA Style

    Huang Liu, Yi Chen. Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks. Sci Discov. 2019;7(3):147-151. doi: 10.11648/j.sd.20190703.13

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  • @article{10.11648/j.sd.20190703.13,
      author = {Huang Liu and Yi Chen},
      title = {Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks},
      journal = {Science Discovery},
      volume = {7},
      number = {3},
      pages = {147-151},
      doi = {10.11648/j.sd.20190703.13},
      url = {https://doi.org/10.11648/j.sd.20190703.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20190703.13},
      abstract = {In this study, a simple yet effective protein detection method was established by using bimetallic Au@Ag core-shell nanobrick as SERS active building blocks. The Au@Ag core-shell nanobrick is synthesized by well-defined ‘two-step’ growing method, enabling uniform and controllable silver shell growth on the surface of the gold nanorods induced by surfactant replacement. The thickness of the silver shell can be programmably adjusted by controlling the amount of silver nitrate in the system, which in turn realized the controllable adjustment of the optical properties of Au@Ag core-shell nanobrick. We selected bovine serum albumin as a model protein and established a highly-sensitive assay for label-free SERS detection of protein. The detection limit can reach to as low as 0.5 μg/mL, enabling promising avenue for its clinical application in ultrasensitive biomarker detection.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Label-Free SERS Detection of Bovine Serum Albumin Protein Based on Au@Ag Core/Shell Nanobricks
    AU  - Huang Liu
    AU  - Yi Chen
    Y1  - 2019/06/15
    PY  - 2019
    N1  - https://doi.org/10.11648/j.sd.20190703.13
    DO  - 10.11648/j.sd.20190703.13
    T2  - Science Discovery
    JF  - Science Discovery
    JO  - Science Discovery
    SP  - 147
    EP  - 151
    PB  - Science Publishing Group
    SN  - 2331-0650
    UR  - https://doi.org/10.11648/j.sd.20190703.13
    AB  - In this study, a simple yet effective protein detection method was established by using bimetallic Au@Ag core-shell nanobrick as SERS active building blocks. The Au@Ag core-shell nanobrick is synthesized by well-defined ‘two-step’ growing method, enabling uniform and controllable silver shell growth on the surface of the gold nanorods induced by surfactant replacement. The thickness of the silver shell can be programmably adjusted by controlling the amount of silver nitrate in the system, which in turn realized the controllable adjustment of the optical properties of Au@Ag core-shell nanobrick. We selected bovine serum albumin as a model protein and established a highly-sensitive assay for label-free SERS detection of protein. The detection limit can reach to as low as 0.5 μg/mL, enabling promising avenue for its clinical application in ultrasensitive biomarker detection.
    VL  - 7
    IS  - 3
    ER  - 

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Author Information
  • State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science & Medical Engineering, Southeast University, Nanjing, China

  • State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science & Medical Engineering, Southeast University, Nanjing, China

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