Hydrogen production yield of photosynthetic bacteria is low, which limits the development of the photo-fermentation. In order to solve this problem, We're trying to over-expression [FeFe]-hydrogenase in photosynthetic bacteria to improve its hydrogen production yield.[FeFe]-hydrogenase, from Rhodopseudomonas palustris CGA009 (RpHydC), Rhodospirillum rubrum ATCC 11170 (RrhydA), Clostridium acetobutylicum ATCC 824(CahydA) are selected as the research object. We construct the over-expression plasmid of pCG2,pAD2 and pCA2,which containing RphydC, RrhydA and CahydA DNA fragment, respectively. The hydG, hydE and hydF are amplified and cloned in pBBR1mcs-2 to form pEFG2. The strains A036 containing pCA2 and pEFG2, A037 containing pCG2 and pEFG2, A038 containing pAD2 and pEFG2.The hydrogen yield of A036, A037 and A038 are 1.38 mol H2/mol-glucose，1.34 mol H2/mol-glucose and 1.23 mol H2/mol-glucose,which compared with wild type strain increased by 29.71%, 25.52% and 15.07%, respectively.In this study, we successful implementation of the [FeFe]-hydrogenase heterologous expression in Rhodobacter sphaeroides HY01 by the test analysis of hydrogen production and RT-PCR. Otherwise, [FeFe]-hydrogenase,encoded by RphydC, RrhydA and CahydA are confirmed that their maturation was strictly dependent on co-expression of hydG, hydE, and hydF.
| Published in | Science Discovery (Volume 10, Issue 3) |
| DOI | 10.11648/j.sd.20221003.25 |
| Page(s) | 173-180 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Heterologous Expression, [FeFe]-hydrogenase, Hydrogen Yield
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APA Style
An Dan, Song Zi-lun, Su Zhen-hua, Wei Hao-wen, Xiang Ming-rui, et al. (2022). Improved Hydrogen Production by Overexpression of RphydC, RrhydA, CahydA in Rhodobacter sphaeroides HY01. Science Discovery, 10(3), 173-180. https://doi.org/10.11648/j.sd.20221003.25
ACS Style
An Dan; Song Zi-lun; Su Zhen-hua; Wei Hao-wen; Xiang Ming-rui, et al. Improved Hydrogen Production by Overexpression of RphydC, RrhydA, CahydA in Rhodobacter sphaeroides HY01. Sci. Discov. 2022, 10(3), 173-180. doi: 10.11648/j.sd.20221003.25
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Download@article{10.11648/j.sd.20221003.25,
author = {An Dan and Song Zi-lun and Su Zhen-hua and Wei Hao-wen and Xiang Ming-rui and Yin Yue},
title = {Improved Hydrogen Production by Overexpression of RphydC, RrhydA, CahydA in Rhodobacter sphaeroides HY01},
journal = {Science Discovery},
volume = {10},
number = {3},
pages = {173-180},
doi = {10.11648/j.sd.20221003.25},
url = {https://doi.org/10.11648/j.sd.20221003.25},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20221003.25},
abstract = {Hydrogen production yield of photosynthetic bacteria is low, which limits the development of the photo-fermentation. In order to solve this problem, We're trying to over-expression [FeFe]-hydrogenase in photosynthetic bacteria to improve its hydrogen production yield.[FeFe]-hydrogenase, from Rhodopseudomonas palustris CGA009 (RpHydC), Rhodospirillum rubrum ATCC 11170 (RrhydA), Clostridium acetobutylicum ATCC 824(CahydA) are selected as the research object. We construct the over-expression plasmid of pCG2,pAD2 and pCA2,which containing RphydC, RrhydA and CahydA DNA fragment, respectively. The hydG, hydE and hydF are amplified and cloned in pBBR1mcs-2 to form pEFG2. The strains A036 containing pCA2 and pEFG2, A037 containing pCG2 and pEFG2, A038 containing pAD2 and pEFG2.The hydrogen yield of A036, A037 and A038 are 1.38 mol H2/mol-glucose，1.34 mol H2/mol-glucose and 1.23 mol H2/mol-glucose,which compared with wild type strain increased by 29.71%, 25.52% and 15.07%, respectively.In this study, we successful implementation of the [FeFe]-hydrogenase heterologous expression in Rhodobacter sphaeroides HY01 by the test analysis of hydrogen production and RT-PCR. Otherwise, [FeFe]-hydrogenase,encoded by RphydC, RrhydA and CahydA are confirmed that their maturation was strictly dependent on co-expression of hydG, hydE, and hydF.},
year = {2022}
}
TY - JOUR T1 - Improved Hydrogen Production by Overexpression of RphydC, RrhydA, CahydA in Rhodobacter sphaeroides HY01 AU - An Dan AU - Song Zi-lun AU - Su Zhen-hua AU - Wei Hao-wen AU - Xiang Ming-rui AU - Yin Yue Y1 - 2022/06/09 PY - 2022 N1 - https://doi.org/10.11648/j.sd.20221003.25 DO - 10.11648/j.sd.20221003.25 T2 - Science Discovery JF - Science Discovery JO - Science Discovery SP - 173 EP - 180 PB - Science Publishing Group SN - 2331-0650 UR - https://doi.org/10.11648/j.sd.20221003.25 AB - Hydrogen production yield of photosynthetic bacteria is low, which limits the development of the photo-fermentation. In order to solve this problem, We're trying to over-expression [FeFe]-hydrogenase in photosynthetic bacteria to improve its hydrogen production yield.[FeFe]-hydrogenase, from Rhodopseudomonas palustris CGA009 (RpHydC), Rhodospirillum rubrum ATCC 11170 (RrhydA), Clostridium acetobutylicum ATCC 824(CahydA) are selected as the research object. We construct the over-expression plasmid of pCG2,pAD2 and pCA2,which containing RphydC, RrhydA and CahydA DNA fragment, respectively. The hydG, hydE and hydF are amplified and cloned in pBBR1mcs-2 to form pEFG2. The strains A036 containing pCA2 and pEFG2, A037 containing pCG2 and pEFG2, A038 containing pAD2 and pEFG2.The hydrogen yield of A036, A037 and A038 are 1.38 mol H2/mol-glucose，1.34 mol H2/mol-glucose and 1.23 mol H2/mol-glucose,which compared with wild type strain increased by 29.71%, 25.52% and 15.07%, respectively.In this study, we successful implementation of the [FeFe]-hydrogenase heterologous expression in Rhodobacter sphaeroides HY01 by the test analysis of hydrogen production and RT-PCR. Otherwise, [FeFe]-hydrogenase,encoded by RphydC, RrhydA and CahydA are confirmed that their maturation was strictly dependent on co-expression of hydG, hydE, and hydF. VL - 10 IS - 3 ER -
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