Polyporus umbellatus is a kind of medicine and food fungus, in addition to the main component of polysaccharides, steroid components are rich in content, good biological activity and small side effects, part of its efficacy has been widely used in the treatment of various diseases. To investigate the anti-tumor biological activity and pharmacological effects of the steroid components, thiazole blue (MTT) method was used to evaluate the anti-proliferative effect of Polyporus umbellatus steroids on human hepatocarcinoma cell line HepG2, human cervical carcinoma cell line Hela, human renal clear-cell carcinoma cell line 786-O and human renal proximal tubular epithelial cell line HKC. The results indicated that different steroid compounds at different concentrations showed different degrees of inhibition of cell proliferation, as the concentration of the compound increases, its inhibition rate gradually increases, and it is concentration-dependent. The inhibitory effect of different compounds on the proliferation of the same cell was also different, among which the IC50 value of compound ZT-5 on HepG2 cells, Hela cells and 786-O cells was only 14.36 μg/mL, 21.90 μg/mL and 46.56 μg/mL, far lower than that of other compounds. The cytotoxic activity of Polyporus umbellatus steroids against tumor cells HepG2 and Hela was higher than that of 786-O cells, and the cytotoxic activity against normal cells HKC was less, showing obvious cell selectivity. The Polyporus umbellatus steroids have obvious anti-proliferative effect on tumor cells, which expand the therapeutic potential and have important guiding significance for the clinical application of Polyporus umbellatus.
| Published in | Science Discovery (Volume 10, Issue 3) |
| DOI | 10.11648/j.sd.20221003.17 |
| Page(s) | 125-131 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
MTT Method, Polyporus umbellatus, Steroids, Anti-tumor
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APA Style
Zhang Hai-xiang, Hong Dong-feng, Zhao Xiang-rong, Wang Jin-ping, Guo Hua. (2022). MTT Method for Detecting the Anti-tumor Effect of Polyporus umbellatus Steroids. Science Discovery, 10(3), 125-131. https://doi.org/10.11648/j.sd.20221003.17
ACS Style
Zhang Hai-xiang; Hong Dong-feng; Zhao Xiang-rong; Wang Jin-ping; Guo Hua. MTT Method for Detecting the Anti-tumor Effect of Polyporus umbellatus Steroids. Sci. Discov. 2022, 10(3), 125-131. doi: 10.11648/j.sd.20221003.17
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Download@article{10.11648/j.sd.20221003.17,
author = {Zhang Hai-xiang and Hong Dong-feng and Zhao Xiang-rong and Wang Jin-ping and Guo Hua},
title = {MTT Method for Detecting the Anti-tumor Effect of Polyporus umbellatus Steroids},
journal = {Science Discovery},
volume = {10},
number = {3},
pages = {125-131},
doi = {10.11648/j.sd.20221003.17},
url = {https://doi.org/10.11648/j.sd.20221003.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20221003.17},
abstract = {Polyporus umbellatus is a kind of medicine and food fungus, in addition to the main component of polysaccharides, steroid components are rich in content, good biological activity and small side effects, part of its efficacy has been widely used in the treatment of various diseases. To investigate the anti-tumor biological activity and pharmacological effects of the steroid components, thiazole blue (MTT) method was used to evaluate the anti-proliferative effect of Polyporus umbellatus steroids on human hepatocarcinoma cell line HepG2, human cervical carcinoma cell line Hela, human renal clear-cell carcinoma cell line 786-O and human renal proximal tubular epithelial cell line HKC. The results indicated that different steroid compounds at different concentrations showed different degrees of inhibition of cell proliferation, as the concentration of the compound increases, its inhibition rate gradually increases, and it is concentration-dependent. The inhibitory effect of different compounds on the proliferation of the same cell was also different, among which the IC50 value of compound ZT-5 on HepG2 cells, Hela cells and 786-O cells was only 14.36 μg/mL, 21.90 μg/mL and 46.56 μg/mL, far lower than that of other compounds. The cytotoxic activity of Polyporus umbellatus steroids against tumor cells HepG2 and Hela was higher than that of 786-O cells, and the cytotoxic activity against normal cells HKC was less, showing obvious cell selectivity. The Polyporus umbellatus steroids have obvious anti-proliferative effect on tumor cells, which expand the therapeutic potential and have important guiding significance for the clinical application of Polyporus umbellatus.},
year = {2022}
}
TY - JOUR T1 - MTT Method for Detecting the Anti-tumor Effect of Polyporus umbellatus Steroids AU - Zhang Hai-xiang AU - Hong Dong-feng AU - Zhao Xiang-rong AU - Wang Jin-ping AU - Guo Hua Y1 - 2022/06/01 PY - 2022 N1 - https://doi.org/10.11648/j.sd.20221003.17 DO - 10.11648/j.sd.20221003.17 T2 - Science Discovery JF - Science Discovery JO - Science Discovery SP - 125 EP - 131 PB - Science Publishing Group SN - 2331-0650 UR - https://doi.org/10.11648/j.sd.20221003.17 AB - Polyporus umbellatus is a kind of medicine and food fungus, in addition to the main component of polysaccharides, steroid components are rich in content, good biological activity and small side effects, part of its efficacy has been widely used in the treatment of various diseases. To investigate the anti-tumor biological activity and pharmacological effects of the steroid components, thiazole blue (MTT) method was used to evaluate the anti-proliferative effect of Polyporus umbellatus steroids on human hepatocarcinoma cell line HepG2, human cervical carcinoma cell line Hela, human renal clear-cell carcinoma cell line 786-O and human renal proximal tubular epithelial cell line HKC. The results indicated that different steroid compounds at different concentrations showed different degrees of inhibition of cell proliferation, as the concentration of the compound increases, its inhibition rate gradually increases, and it is concentration-dependent. The inhibitory effect of different compounds on the proliferation of the same cell was also different, among which the IC50 value of compound ZT-5 on HepG2 cells, Hela cells and 786-O cells was only 14.36 μg/mL, 21.90 μg/mL and 46.56 μg/mL, far lower than that of other compounds. The cytotoxic activity of Polyporus umbellatus steroids against tumor cells HepG2 and Hela was higher than that of 786-O cells, and the cytotoxic activity against normal cells HKC was less, showing obvious cell selectivity. The Polyporus umbellatus steroids have obvious anti-proliferative effect on tumor cells, which expand the therapeutic potential and have important guiding significance for the clinical application of Polyporus umbellatus. VL - 10 IS - 3 ER -
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